• Product name

    Anti-RNF11 antibody [EPR6820]
    See all RNF11 primary antibodies
  • Description

    Rabbit monoclonal [EPR6820] to RNF11
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human RNF11 (UniProt Q9Y3C5).

  • Positive control

    • MCF7 and Human fetal liver lysates; Human brain and kidney tissues; MCF7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab154831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.
    • Tissue specificity

      Expressed at low levels in the lung, liver, kidney, pancreas, spleen, prostate, thymus, ovary, small intestine, colon, and peripheral blood lymphocytes, and, at intermediate levels, in the testis, heart, brain and placenta. Highest expression in the skeletal muscle. In the brain, expressed at different levels in several regions: high levels in the amygdala, moderate in the hippocampus and thalamus, low in the caudate and extremely low levels in the corpus callosum (at protein level). Restricted to neurons, enriched in somatodendritic compartments and excluded from white matter (at protein level). In substantia nigra, present in cell bodies and processes of dopaminergic and nondopaminergic cells (at protein level). In Parkinson disease, sequestered in Lewy bodies and neurites. Overexpressed in breast cancer cells, but not detected in the surrounding stroma and weakly, if at all, in normal breast epithelial cells (at protein level). Also expressed in several tumor cell lines.
    • Sequence similarities

      Contains 1 RING-type zinc finger.
    • Domain

      The WW-binding motif mediates interaction with NEDD4.
    • Post-translational

      Ubiquitinated in the presence of SMURF2 and UBE2D1, as well as WWP1.
      Phosphorylation by PKB/AKT1 may accelerate degradation by the proteasome.
    • Cellular localization

      Cytoplasm. Nucleus. Predominantly cytoplasmic, when unphosphorylated, and nuclear, when phosphorylated by PKB/AKT1.
    • Information by UniProt
    • Database links

    • Alternative names

      • CGI 123 antibody
      • RING finger protein 11 antibody
      • RNF11 antibody
      • RNF11_HUMAN antibody
      • Sid 1669 antibody
      • SID1669 antibody
      see all


    • All lanes : Anti-RNF11 antibody [EPR6820] (ab154831) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RNF11 with ab154831 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RNF11 with ab154831 at 1/50 dilution.
    • Flow cytometric analysis of permeabilized MCF7 cells labeling RNF11 with ab154831 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).


    This product has been referenced in:

    • Meng Z  et al. A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination. J Immunol 198:820-831 (2017). Read more (PubMed: 27986908) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Western blot
    Human Cell lysate - whole cell (H1, Hues1, MCF7, HEK 293, Hela)
    Gel Running Conditions
    Reduced Denaturing (12% polyacrylamide gel)
    Loading amount
    30 µg
    H1, Hues1, MCF7, HEK 293, Hela
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5%

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    Submitted Aug 08 2016

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