Recombinant Anti-RNF115 antibody [EPR14539] (ab187642)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14539] to RNF115
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RNF115 antibody [EPR14539]
See all RNF115 primary antibodies -
Description
Rabbit monoclonal [EPR14539] to RNF115 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain lysate; PC-3, Hela, Raw264.7, PC-12 and NIH/3T3 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14539 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/2000. Predicted molecular weight: 34 kDa.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 34 kDa. |
Target
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Function
Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking. -
Tissue specificity
Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Post-translational
modificationsRING-type zinc finger-dependent and E2-dependent autoubiquitination. -
Cellular localization
Cytoplasm > cytosol. The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes. - Information by UniProt
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Database links
- Entrez Gene: 101060478 Human
- Entrez Gene: 27246 Human
- Entrez Gene: 67845 Mouse
- Entrez Gene: 362002 Rat
- SwissProt: Q9Y4L5 Human
- SwissProt: Q9D0C1 Mouse
- Unigene: 523550 Human
- Unigene: 386792 Mouse
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Alternative names
- BCA2 antibody
- Breast cancer associated gene 2 antibody
- CL469780 antibody
see all
Images
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All lanes : Anti-RNF115 antibody [EPR14539] (ab187642) at 1/1000 dilution
Lane 1 : RAW 264.7 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa -
All lanes : Anti-RNF115 antibody [EPR14539] (ab187642) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa -
Anti-RNF115 antibody [EPR14539] (ab187642) at 1/1000 dilution + PC-3 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab187642 has been referenced in 1 publication.
- Zhang Q et al. A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. Theranostics 10:10769-10790 (2020). PubMed: 32929379