Key features and details
- Rabbit polyclonal to RNF128/GRAIL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Horse, Dog, Human, Chimpanzee, Monkey
- Isotype: IgG
Product nameAnti-RNF128/GRAIL antibody
See all RNF128/GRAIL primary antibodies
DescriptionRabbit polyclonal to RNF128/GRAIL
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Horse, Dog, Human, Chimpanzee, Monkey
Predicted to work with: Chicken, Cow, Opossum, Orangutan
- Human, mouse and rat spleen lysates. Human brain tissue.
Previously labelled as RNF128.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab72533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
FunctionE3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 PA (protease associated) domain.
Contains 1 RING-type zinc finger.
DomainBinding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
modificationsAuto-ubiquitinated. Controls the development of T cell clonal anergy by ubiquitination.
Cellular localizationEndomembrane system. Cytoplasm > perinuclear region. Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7.
- Information by UniProt
- E3 ubiquitin protein ligase RNF128 antibody
- E3 ubiquitin-protein ligase RNF128 antibody
- FLJ23516 antibody
All lanes : Anti-RNF128/GRAIL antibody (ab72533) at 1 µg/ml
Lane 1 : human spleen lysate
Lane 2 : human spleen lysate with immunising peptide
Lane 3 : mouse spleen lysate
Lane 4 : rat spleen lysate
Predicted band size: 47 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Endogenous RNF128 / GRAIL is reported to be detected at approximately 66 kD
Immunohistochemical staining of paraffin-embedded normal human brain tissue using ab72533 at 5 ug/ml (Panel A) and control rabbit Ig at 5 ug/ml (Panel B).
ab72533 has not yet been referenced specifically in any publications.