Overview

  • Product name

    Anti-RNF128/GRAIL antibody [EPR7426(2)]
    See all RNF128/GRAIL primary antibodies
  • Description

    Rabbit monoclonal [EPR7426(2)] to RNF128/GRAIL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human RNF128/GRAIL aa 350 to the C-terminus.
    Database link: Q8TEB7

  • Positive control

    • WB: HeLa, 293T, U-87 MG, RAW 264.7 and NIH 3T3 cell lysates
  • General notes

     

     

    Previously labelled as RNF128. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7426(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 PA (protease associated) domain.
      Contains 1 RING-type zinc finger.
    • Domain

      Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
    • Post-translational
      modifications

      Auto-ubiquitinated. Controls the development of T cell clonal anergy by ubiquitination.
    • Cellular localization

      Endomembrane system. Cytoplasm > perinuclear region. Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 ubiquitin protein ligase RNF128 antibody
      • E3 ubiquitin-protein ligase RNF128 antibody
      • FLJ23516 antibody
      • Gene related to anergy in lymphocytes protein antibody
      • GRAIL antibody
      • RING finger protein 128 antibody
      • RN128_HUMAN antibody
      • RNF128 antibody
      see all

    Images

    • All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

      Lane 1 : RAW264.7 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 46 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 46 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : U-87 MG cell lysates
      Lane 4 : RAW 264.7 cell lysates
      Lane 5 : NIH 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 47 kDa

    References

    This product has been referenced in:

    • Delpoux A  et al. Continuous activity of Foxo1 is required to prevent anergy and maintain the memory state of CD8+ T cells. J Exp Med 215:575-594 (2018). Read more (PubMed: 29282254) »
    • Aziz M  et al. Upregulation of GRAIL is associated with impaired CD4 T cell proliferation in sepsis. J Immunol 192:2305-14 (2014). Read more (PubMed: 24477910) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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