Overview

  • Product name
  • Description
    Goat polyclonal to RNF13
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: PNGERDYNIANTV, corresponding to C terminal amino acids 369-381 of Human RNF13.

  • Positive control
    • Human pancreas.
  • General notes
    Principal Names – RNF13; RZF; ring finger protein 13; RING zinc finger protein. Official Gene Symbol - RNF13. GenBank Accession Number – NP_009213. LocusLink ID - 11342 (human). Gene Ontology terms - peptidase activity; proteolysis and peptidolysis; nucleus.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. This antibodoidy stained islets but not in ducts and acini of Human normal pancreas. However, in pancreatic ductal adenocarcinoma staining was in the ducts and acini.
WB Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human RNF13 peptide (ab23020).

Target

  • Function
    E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.
  • Tissue specificity
    Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain is required for E3 ligase activity.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase RNF13 antibody
    • FLJ93817 antibody
    • MGC13689 antibody
    • RING finger protein 13 antibody
    • Ring zinc finger protein antibody
    • RNF 13 antibody
    • Rnf13 antibody
    • RNF13_HUMAN antibody
    • RZF antibody
    see all

References

This product has been referenced in:
  • Zhang Q  et al. RNF13: a novel RING-type ubiquitin ligase over-expressed in pancreatic cancer. Cell Res 19:348-57 (2009). WB, IHC-P ; Human . Read more (PubMed: 18794910) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - Triton
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde

Miss. Chen Avrahami

Verified customer

Submitted May 16 2016

Answer

Thank you very much for contacting us regarding ab4288. I am very sorry to hear that you are experiencing difficulty with this antibody. Where did you place the tag on RNF13? It is possible that the tag is interfering with your ability to detect the overexpressed RNF13 especially if the tag is on the C-terminus. This is also theoretically possible if the tag is on the N-terminus but is not as easy to explain. I would recommend moving the epitope tag or using a different tag and then see what result you have. The predicted molecular weight of the unprocessed precursor RNF13 is 42.8 kDa. If it is processed then the MW will be slightly lower. While the antibody has worked for us in a peptide ELISA, we have not observed a band in WB so I cannot tell you the actual detected MW. As you may know, ab4288 is one of our Fast-track antibodies and it is not yet fully characterized. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. However, Fast-Track antibodies are offered at a reduced price. We would be very grateful for you if you could submit a review via the product datasheet. We have a very special Feedback reward system for Fast-track products: we would greatly appreciate your feedback on this antibody, whether positive or negative. We will award 950 Abpoints to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 950 Abpoints. Other useful positive and negative feedback will be rewarded with 50 Abpoints. To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Earn Abpoints section on the product datasheet. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com and would be grateful if you could also attach your Western blot image. We hope to receive your review very shortly. Thank you for your time and your effort,

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