Anti-RNF13 antibody - C-terminal (ab151601)
Key features and details
- Rabbit polyclonal to RNF13 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF13 antibody - C-terminal -
Description
Rabbit polyclonal to RNF13 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to a region within amino acids 319 and 381 of RNF13 (Uniprot ID: O43567)
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji whole cell lysate; MCF7 cells; A549 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation. -
Tissue specificity
Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 PA (protease associated) domain.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger domain is required for E3 ligase activity. -
Post-translational
modificationsAuto-ubiquitinated. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane. - Information by UniProt
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Database links
- Entrez Gene: 11342 Human
- Omim: 609247 Human
- SwissProt: Q0VD51 Cow
- SwissProt: O43567 Human
- Unigene: 12333 Human
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Alternative names
- E3 ubiquitin-protein ligase RNF13 antibody
- FLJ93817 antibody
- MGC13689 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded A549 xenograft, labeling RNF13 using ab151601 at 1/500 dilution.
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Anti-RNF13 antibody - C-terminal (ab151601) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed MCF7, labeling RNF13 using ab151601 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151601 has been referenced in 1 publication.
- Stamataki D et al. Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system. PLoS One 6:e24484 (2011). IHC . PubMed: 21915337