Overview

  • Product name

    Anti-RNF13 antibody - C-terminal
    See all RNF13 primary antibodies
  • Description

    Rabbit polyclonal to RNF13 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 319 and 381 of RNF13 (Uniprot ID: O43567)

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji whole cell lysate; MCF7 cells; A549 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab151601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.
  • Tissue specificity

    Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain is required for E3 ligase activity.
  • Post-translational
    modifications

    Auto-ubiquitinated.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase RNF13 antibody
    • FLJ93817 antibody
    • MGC13689 antibody
    • RING finger protein 13 antibody
    • Ring zinc finger protein antibody
    • RNF 13 antibody
    • Rnf13 antibody
    • RNF13_HUMAN antibody
    • RZF antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, labeling RNF13 using ab151601 at 1/500 dilution.
  • Anti-RNF13 antibody - C-terminal (ab151601) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed MCF7, labeling RNF13 using ab151601 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Stamataki D  et al. Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system. PLoS One 6:e24484 (2011). IHC . Read more (PubMed: 21915337) »
See 1 Publication for this product

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