Product nameAnti-RNF130 antibody
DescriptionRabbit polyclonal to RNF130
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Fetal thymus lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab49218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:62500.
|WB||Use a concentration of 2.5 mg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceRNF130 contains a RING finger motif and is similar to g1, a Drosophila zinc-finger protein that is expressed in mesoderm and involved in embryonic development. The expression of the mouse counterpart was found to be upregulated in myeloblastic cells following IL3 deprivation, suggesting that this gene may regulate growth factor withdrawal-induced apoptosis of myeloid precursor cells.
Cellular localizationMembrane; single-pass type I membrane protein
- 2510042A13Rik antibody
- G1 related zinc finger protein antibody
- G1rp antibody
Anti-RNF130 antibody (ab49218) at 2.5 µg/ml + Fetal thymus lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
ab49218 has not yet been referenced specifically in any publications.