Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||1/250 - 1/500. Predicted molecular weight: 48 kDa.|
FunctionActs as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production.
Tissue specificityExpressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in RNF135 are the cause of macrocephaly macrosomia facial dysmorphism syndrome (MMFD) [MIM:614192]. MMFD is an autosomal dominant disorder characterized by the association of macrothrombocytopathy and progressive sensorineural hearing loss without renal dysfunction.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- E3 ubiquitin-protein ligase RNF135 antibody
- L13 antibody
- MGC13061 antibody
All lanes : Anti-RNF135 antibody (ab220414) at 1/250 dilution
Lane 1 : human RT-4 cell line
Lane 2 : human U-251 MG cell line
Lane 3 : human plasma tissue
Lane 4 : human liver tissue
Predicted band size: 48 kDa
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cell line labeling RNF135 with ab220414 at 4 µg/ml (green).
ab220414 has not yet been referenced specifically in any publications.