Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF135
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RNF135 aa 353-432.
    Sequence:

    GTSQLSAWHMVKETVLGSDRPGVVGIWLNLEEGKLAFYSVDNQEKLLYEC TISASSPLYPAFWLYGLHPGNYLIIKQVKV


    Database link: Q8IUD6

  • Positive control

    • Human plasma tissue; A431 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab220414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

WB 1/250 - 1/500. Predicted molecular weight: 48 kDa.

Target

  • Function

    Acts as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production.
  • Tissue specificity

    Expressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Defects in RNF135 are the cause of macrocephaly macrosomia facial dysmorphism syndrome (MMFD) [MIM:614192]. MMFD is an autosomal dominant disorder characterized by the association of macrothrombocytopathy and progressive sensorineural hearing loss without renal dysfunction.
  • Sequence similarities

    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase RNF135 antibody
    • L13 antibody
    • MGC13061 antibody
    • MMFD antibody
    • REUL antibody
    • RIG-I E3 ubiquitin ligase antibody
    • RING finger protein 135 antibody
    • Riplet antibody
    • RN135_HUMAN antibody
    • Rnf135 antibody
    see all

Images

  • All lanes : Anti-RNF135 antibody (ab220414) at 1/250 dilution

    Lane 1 : human RT-4 cell line
    Lane 2 : human U-251 MG cell line
    Lane 3 : human plasma tissue
    Lane 4 : human liver tissue

    Predicted band size: 48 kDa

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cell line labeling RNF135 with ab220414 at 4 µg/ml (green).

References

ab220414 has not yet been referenced specifically in any publications.

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