Key features and details
- Rabbit polyclonal to RNF135
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF135 antibody
See all RNF135 primary antibodies
DescriptionRabbit polyclonal to RNF135
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: HEK-293T and Jurkat whole cell lysates. IHC-P: Human gastric cancer and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionActs as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production.
Tissue specificityExpressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in RNF135 are the cause of macrocephaly macrosomia facial dysmorphism syndrome (MMFD) [MIM:614192]. MMFD is an autosomal dominant disorder characterized by the association of macrothrombocytopathy and progressive sensorineural hearing loss without renal dysfunction.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- E3 ubiquitin-protein ligase RNF135 antibody
- L13 antibody
- MGC13061 antibody
All lanes : Anti-RNF135 antibody (ab229959) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Paraffin-embedded human gastric cancer tissue stained for RNF135 using ab229959 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for RNF135 using ab229959 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229959 has not yet been referenced specifically in any publications.