Overview

  • Product name

    Anti-RNF138/HSD-4 antibody
    See all RNF138/HSD-4 primary antibodies
  • Description

    Rabbit polyclonal to RNF138/HSD-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human RNF138/HSD-4 (internal sequence).

  • Positive control

    • MCF7 cell lysate
  • General notes

     This product was previously labelled as RNF138

     

Properties

Applications

Our Abpromise guarantee covers the use of ab92730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 RING-type zinc finger.
    • Domain

      The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    • Post-translational
      modifications

      Auto-ubiquitinated.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 ubiquitin protein ligase RNF138 antibody
      • E3 ubiquitin-protein ligase RNF138 antibody
      • hNARF antibody
      • HSD 4 antibody
      • HSD4 antibody
      • MGC8758 antibody
      • NARF antibody
      • Nemo like kinase associated RING finger protein antibody
      • Nemo-like kinase-associated RING finger protein antibody
      • NLK associated RING finger protein antibody
      • NLK-associated RING finger protein antibody
      • RING finger protein 138 antibody
      • ring finger protein 138, E3 ubiquitin protein ligase antibody
      • RN138_HUMAN antibody
      • Rnf138 antibody
      • STRIN antibody
      see all

    Images

    • All lanes : Anti-RNF138/HSD-4 antibody (ab92730) at 1/500 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : MCF7 cell lysate with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    References

    This product has been referenced in:

    • Fu SJ  et al. Ubiquitin Ligase RNF138 Promotes Episodic Ataxia Type 2-Associated Aberrant Degradation of Human Cav2.1 (P/Q-Type) Calcium Channels. J Neurosci 37:2485-2503 (2017). Read more (PubMed: 28167673) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Multiple Tissues)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount
    30 µg
    Specification
    Multiple Tissues
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up