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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase
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RecombinantRabMAb

Recombinant Anti-RNF14 antibody [EPR9041] (ab134927)

  • Datasheet
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Abpromise

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Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)
  • Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)
  • OI-RD Scanning - Anti-RNF14 antibody [EPR9041] (ab134927)
  • Anti-RNF14 antibody [EPR9041] (ab134927)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9041] to RNF14
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RNF14 antibody [EPR9041]
    See all RNF14 primary antibodies
  • Description

    Rabbit monoclonal [EPR9041] to RNF14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RNF14 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • LNCaP, K562, Jurkat, and HeLa whole cell lysate (ab150035). Permeabilized K562 cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 2.18 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9041
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Alternative Versions

    • Anti-RNF14 antibody [EPR9041] - BSA and Azide free (ab248736)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
    • K562 whole cell lysate (ab7911)

Applications

Our Abpromise guarantee covers the use of ab134927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.
    • Tissue specificity

      Widely expressed.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the RBR family. RNF14 subfamily.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
      Contains 1 RWD domain.
    • Domain

      The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
      The RING-type zinc finger is essential for the interaction with UBE2E2.
    • Post-translational
      modifications

      RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession Q9UBS8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9604 Human
      • Entrez Gene: 56736 Mouse
      • Entrez Gene: 619577 Rat
      • Omim: 605675 Human
      • SwissProt: Q9UBS8 Human
      • SwissProt: Q9JI90 Mouse
      • Unigene: 483616 Human
      • Unigene: 228903 Mouse
      • Unigene: 115357 Rat
      see all
    • Alternative names

      • Androgen receptor associated protein 54 antibody
      • Androgen receptor-associated protein 54 antibody
      • ARA 54 antibody
      • ARA54 antibody
      • E3 ubiquitin protein ligase RNF14 antibody
      • E3 ubiquitin-protein ligase RNF14 antibody
      • FLJ26004 antibody
      • HFB 30 antibody
      • HFB30 antibody
      • HRIHFB2038 antibody
      • RING finger protein 14 antibody
      • RNF 14 antibody
      • Rnf14 antibody
      • RNF14_HUMAN antibody
      • TRIAD 2 antibody
      • TRIAD2 antibody
      • Triad2 protein antibody
      see all

    Images

    • Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)
      Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)
      All lanes : Anti-RNF14 antibody [EPR9041] (ab134927) at 1/1000 dilution

      Lane 1 : LNCaP cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    • Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)
      Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)
      Flow cytometric analysis of permeabilized K562 cells labelling RNF14 with ab134927 at 1/10 dilution (red) or a non-specific rabbit IgG (green).
    • OI-RD Scanning - Anti-RNF14 antibody [EPR9041] (ab134927)
      OI-RD Scanning - Anti-RNF14 antibody [EPR9041] (ab134927)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-RNF14 antibody [EPR9041] (ab134927)
      Anti-RNF14 antibody [EPR9041] (ab134927)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab134927? Please let us know so that we can cite the reference in this datasheet.

    ab134927 has been referenced in 1 publication.

    • Polajnar M  et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab134927.
    Please use the links above to contact us or submit feedback about this product.

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