Recombinant Anti-RNF14 antibody [EPR9041] (ab134927)
![Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-149097-anti-rnf14-antibody-epr9041-western-blot.jpg "Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9041] to RNF14
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RNF14 antibody [EPR9041]
See all RNF14 primary antibodies -
Description
Rabbit monoclonal [EPR9041] to RNF14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RNF14 aa 350-450. The exact sequence is proprietary.
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Positive control
- LNCaP, K562, Jurkat, and HeLa whole cell lysate (ab150035). Permeabilized K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 2.18 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9041 -
Isotype
IgG -
Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab134927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 54 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the RBR family. RNF14 subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 RWD domain. -
Domain
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
The RING-type zinc finger is essential for the interaction with UBE2E2. -
Post-translational
modificationsRING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9604 Human
- Entrez Gene: 56736 Mouse
- Entrez Gene: 619577 Rat
- Omim: 605675 Human
- SwissProt: Q9UBS8 Human
- SwissProt: Q9JI90 Mouse
- Unigene: 483616 Human
- Unigene: 228903 Mouse
see all -
Alternative names
- Androgen receptor associated protein 54 antibody
- Androgen receptor-associated protein 54 antibody
- ARA 54 antibody
see all
Images
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Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)All lanes : Anti-RNF14 antibody [EPR9041] (ab134927) at 1/1000 dilution
Lane 1 : LNCaP cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa -
![Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-149096-anti-rnf14-antibody-epr9041-flow-cytometry.jpg)
Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)Flow cytometric analysis of permeabilized K562 cells labelling RNF14 with ab134927 at 1/10 dilution (red) or a non-specific rabbit IgG (green). -
![OI-RD Scanning - Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-149095-anti-rnf14-antibody-epr9041.jpg)
OI-RD Scanning - Anti-RNF14 antibody [EPR9041] (ab134927) -
![Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-4-benefits-of-recombinant-antibodies.png)
Anti-RNF14 antibody [EPR9041] (ab134927)
Datasheets and documents
References (1)
ab134927 has been referenced in 1 publication.
- Polajnar M et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601
