Recombinant
RabMAb

Recombinant Anti-RNF14 antibody [EPR9041] (ab134927)

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Overview

  • Product name

    Anti-RNF14 antibody [EPR9041]
    See all RNF14 primary antibodies

  • Description

    Rabbit monoclonal [EPR9041] to RNF14

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human RNF14 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • LNCaP, K562, Jurkat, and HeLa whole cell lysate (ab150035). Permeabilized K562 cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.

    • Tissue specificity

      Widely expressed.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the RBR family. RNF14 subfamily.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
      Contains 1 RWD domain.

    • Domain

      The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
      The RING-type zinc finger is essential for the interaction with UBE2E2.

    • Post-translational
      modifications

      RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Androgen receptor associated protein 54 antibody
      • Androgen receptor-associated protein 54 antibody
      • ARA 54 antibody
      • ARA54 antibody
      • E3 ubiquitin protein ligase RNF14 antibody
      • E3 ubiquitin-protein ligase RNF14 antibody
      • FLJ26004 antibody
      • HFB 30 antibody
      • HFB30 antibody
      • HRIHFB2038 antibody
      • RING finger protein 14 antibody
      • RNF 14 antibody
      • Rnf14 antibody
      • RNF14_HUMAN antibody
      • TRIAD 2 antibody
      • TRIAD2 antibody
      • Triad2 protein antibody
      see all

    Images

    • Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)
      Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)
      All lanes : Anti-RNF14 antibody [EPR9041] (ab134927) at 1/1000 dilution

      Lane 1 : LNCaP cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    • Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)
      Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)
      Flow cytometric analysis of permeabilized K562 cells labelling RNF14 with ab134927 at 1/10 dilution (red) or a non-specific rabbit IgG (green).
    • Other - Anti-RNF14 antibody [EPR9041] (ab134927)
      Other - Anti-RNF14 antibody [EPR9041] (ab134927)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

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