Product nameAnti-RNF14 antibody [EPR9041]
See all RNF14 primary antibodies
DescriptionRabbit monoclonal [EPR9041] to RNF14
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RNF14 aa 350-450. The exact sequence is proprietary.
- LNCaP, K562, Jurkat, and HeLa whole cell lysate (ab150035). Permeabilized K562 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 2.18 x 10 -10 M Learn more about KD
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab134927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 54 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMight act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RBR family. RNF14 subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 RWD domain.
DomainThe N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
The RING-type zinc finger is essential for the interaction with UBE2E2.
modificationsRING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Androgen receptor associated protein 54 antibody
- Androgen receptor-associated protein 54 antibody
- ARA 54 antibody
All lanes : Anti-RNF14 antibody [EPR9041] (ab134927) at 1/1000 dilution
Lane 1 : LNCaP cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Flow cytometric analysis of permeabilized K562 cells labelling RNF14 with ab134927 at 1/10 dilution (red) or a non-specific rabbit IgG (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD