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I really am at a loss about where the problem lies with ab75054, because that antibody worked well on endogenously expressed (not transfected) protein the first time I tried it and I was really excited but has subsequently not worked. It has been stored as recommended and also it still works moderately well on transfected protein lysate, but not on endogenously expressed protein in cell lysates. I believe it's a basically a good antibody, but something is not quite right!
Asked on Jul 24 2012