Anti-RNF144A antibody (ab89260)
Key features and details
- Mouse polyclonal to RNF144A
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RNF144A antibody
See all RNF144A primary antibodies -
Description
Mouse polyclonal to RNF144A -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein within Human RNF144A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_055561.2 -
Positive control
- Rat brain tissue lysate, HeLa whole cell lysate (ab150035), RNF144A transfected 293T cell lysate and HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa. |
Target
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Function
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the RBR family. RNF144 subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9781 Human
- Entrez Gene: 500636 Rat
- SwissProt: P50876 Human
- Unigene: 22146 Human
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Alternative names
- KIAA0161 antibody
- Probable E3 ubiquitin protein ligase RNF144A antibody
- Probable E3 ubiquitin-protein ligase RNF144A antibody
see all
Images
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Anti-RNF144A antibody (ab89260) at 5 µg/ml + Rat brain tissue lysate at 50 µg
Predicted band size: 33 kDa -
Anti-RNF144A antibody (ab89260) at 5 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 33 kDa -
All lanes : Anti-RNF144A antibody (ab89260) at 5 µg/ml
Lane 1 : RNF144A transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 33 kDa -
ab89260 at 10µg/ml staining RNF144A on HeLa cell by immunofluorescence
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab89260 has been referenced in 5 publications.
- Han SH & Kim KT RNF144a induces ERK-dependent cell death under oxidative stress via downregulation of vaccinia-related kinase 3. J Cell Sci 133:N/A (2020). PubMed: 33067254
- Ho SR & Lin WC RNF144A sustains EGFR signaling to promote EGF-dependent cell proliferation. J Biol Chem 293:16307-16323 (2018). PubMed: 30171075
- Jin X et al. Targeting glioma stem cells through combined BMI1 and EZH2 inhibition. Nat Med 23:1352-1361 (2017). WB ; Human . PubMed: 29035367
- Ho SR et al. Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain. J Biol Chem 290:23026-38 (2015). PubMed: 26216882
- Ho SR et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). PubMed: 24979766