Overview

  • Product name

    Anti-RNF146 antibody
  • Description

    Rabbit polyclonal to RNF146
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human RNF146 aa 21-188. BC008235.
    Sequence:

    NESCSNTAPS LTVPECAICL QTCVHPVSLP CKHVFCYLCV KGASWLGKRC ALCRQEIPED FLDKPTLLSP EELKAASRGN GEYAWYYEGR NGWWQYDERT SRELEDAFSK GKKNTEMLIA GFLYVADLEN MVQYRRNEHG RRRKIKRDII DIPKKGVAGL RLDCDANT


    Database link: Q9NTX7

  • Positive control

    • HeLa whole cell lysate (ab150035); Human thyroid tissue.

Properties

  • Form

    Lyophilised:Reconstitute in 200 µL sterile H2O.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/200 - 1/1000. Predicted molecular weight: 39 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination.
  • Tissue specificity

    Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Note=Defects in RNF146 are a cause of susceptibility to breast cancer.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
    Contains 1 WWE domain.
  • Domain

    The WWE domain mediates non-covalent poly(ADP-ribose)-binding.
  • Post-translational
    modifications

    Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dactylidin antibody
    • dJ351K20.1 antibody
    • DKFZP434O1427 antibody
    • E3 ubiquitin-protein ligase rnf146 antibody
    • RING finger protein 146 antibody
    • RN146_HUMAN antibody
    • RNF 146 antibody
    • Rnf146 antibody
    • RP3 351K20.1 antibody
    see all

Images

  • Anti-RNF146 antibody (ab201212) at 1/1000 dilution + HeLa cell lysate

    Predicted band size: 39 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid tissue labeling RNF146 with ab201212 at 1/100 dilution.

References

This product has been referenced in:

  • Xu J  et al. NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export. Exp Cell Res 383:111505 (2019). Read more (PubMed: 31326389) »
  • Liu D  et al. Circulating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors. Cell Res 28:918-933 (2018). Read more (PubMed: 30030518) »
See all 2 Publications for this product

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