Key features and details
- Rabbit polyclonal to RNF146
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF146 antibody
DescriptionRabbit polyclonal to RNF146
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan, Xenopus tropicalis
Recombinant fragment corresponding to Human RNF146 aa 21-188. BC008235.
NESCSNTAPS LTVPECAICL QTCVHPVSLP CKHVFCYLCV KGASWLGKRC ALCRQEIPED FLDKPTLLSP EELKAASRGN GEYAWYYEGR NGWWQYDERT SRELEDAFSK GKKNTEMLIA GFLYVADLEN MVQYRRNEHG RRRKIKRDII DIPKKGVAGL RLDCDANT
Database link: Q9NTX7
- HeLa whole cell lysate (ab150035); Human thyroid tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in 200 µL sterile H2O.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab201212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/200 - 1/1000. Predicted molecular weight: 39 kDa.|
FunctionE3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination.
Tissue specificityUbiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseNote=Defects in RNF146 are a cause of susceptibility to breast cancer.
Sequence similaritiesContains 1 RING-type zinc finger.
Contains 1 WWE domain.
DomainThe WWE domain mediates non-covalent poly(ADP-ribose)-binding.
modificationsUbiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
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ab201212 has been referenced in 3 publications.
- Xu J et al. NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export. Exp Cell Res 383:111505 (2019). PubMed: 31326389
- Li N et al. Tankyrase disrupts metabolic homeostasis and promotes tumorigenesis by inhibiting LKB1-AMPK signalling. Nat Commun 10:4363 (2019). PubMed: 31554794
- Liu D et al. Circulating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors. Cell Res 28:918-933 (2018). PubMed: 30030518