Anti-RNF168 antibody (ab151955)
Key features and details
- Rabbit polyclonal to RNF168
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF168 antibody
See all RNF168 primary antibodies -
Description
Rabbit polyclonal to RNF168 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 51-316 of Human RNF168 (Q8IYW5).
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Positive control
- PC3 whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
Target
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Function
E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and ubiquitinates histone H2A and H2AX, leading to amplify the RNF8-dependent H2A ubiquitination and promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in RNF168 are the cause of Riddle syndrome (RIDDLES) [MIM:611943]. Riddle syndrome is characterized by increased radiosensitivity, immunodeficiency, mild motor control and learning difficulties, facial dysmorphism, and short stature. Defects are probably due to impaired localization of TP53BP1 and BRCA1 at DNA lesions. -
Sequence similarities
Belongs to the RNF168 family.
Contains 1 RING-type zinc finger. -
Domain
The MIU motifs (motif interacting with ubiquitin) mediate the interaction with ubiquitin and the localization at sites of DNA damage. -
Cellular localization
Nucleus. Localizes to sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 165918 Human
- Omim: 612688 Human
- SwissProt: Q8IYW5 Human
- Unigene: 250648 Human
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Alternative names
- E3 ubiquitin protein ligase RNF168 antibody
- E3 ubiquitin-protein ligase RNF168 antibody
- FLJ35794 antibody
see all
Images
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Western blot - Anti-RNF168 antibody (ab151955)Anti-RNF168 antibody (ab151955) at 1/1000 dilution + PC3 whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151955 has not yet been referenced specifically in any publications.