Key features and details
- Rabbit polyclonal to RNF169
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RNF169 antibody
See all RNF169 primary antibodies
DescriptionRabbit polyclonal to RNF169
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human RNF169 aa 1-250.
MAAAGPSTRA SSAAAAAALS RRGRRGRCDE TAAAKTGAPG PASGPSLLVL SPPLLQPPLP PRPEESGCAG CLEPPGEAAA LPCGHSLCRG CAQRAADAAG PGCPRCRARG PGWARRRARD DGQADSEVLG ECARRSQPER CRPRRDGGAA AAGPRPEQEP RAAPAEPDFI FRAPIKLSKP GELREEYESL RKLREEKLQE EKPSEDQIHK LLPEDTETGK RKMDEQKKRD EPLVLKTNLE RCPARLSDSE
Database link: Q8NCN4
- RFN169 transfected U2OS, NIH-3T3, INS-1 cell lysates
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab188237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 77 kDa.|
FunctionProbable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RNF169 family.
Contains 1 RING-type zinc finger.
DomainThe MIU motif (motif interacting with ubiquitin) mediates the interaction with both 'Lys-48'- and 'Lys-63'-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction with ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833).
Cellular localizationNucleus > nucleoplasm. Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products.
- Information by UniProt
- E3 ubiquitin-protein ligase RNF169 antibody
- KIAA1991 antibody
- RING finger protein 169 antibody
ab188237 has been referenced in 1 publication.
- R Menon V et al. DYRK1A regulates the recruitment of 53BP1 to the sites of DNA damage in part through interaction with RNF169. Cell Cycle 18:531-551 (2019). WB ; Human . PubMed: 30773093