Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF169
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human RNF169 aa 1-250.
    Sequence:

    MAAAGPSTRA SSAAAAAALS RRGRRGRCDE TAAAKTGAPG PASGPSLLVL SPPLLQPPLP PRPEESGCAG CLEPPGEAAA LPCGHSLCRG CAQRAADAAG PGCPRCRARG PGWARRRARD DGQADSEVLG ECARRSQPER CRPRRDGGAA AAGPRPEQEP RAAPAEPDFI FRAPIKLSKP GELREEYESL RKLREEKLQE EKPSEDQIHK LLPEDTETGK RKMDEQKKRD EPLVLKTNLE RCPARLSDSE


    Database link: Q8NCN4

  • Positive control

    • RFN169 transfected U2OS, NIH-3T3, INS-1 cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 77 kDa.

Target

  • Function

    Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RNF169 family.
    Contains 1 RING-type zinc finger.
  • Domain

    The MIU motif (motif interacting with ubiquitin) mediates the interaction with both 'Lys-48'- and 'Lys-63'-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction with ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833).
  • Cellular localization

    Nucleus > nucleoplasm. Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase RNF169 antibody
    • KIAA1991 antibody
    • RING finger protein 169 antibody
    • RN169_HUMAN antibody
    • RNF169 antibody
    see all

Images

  • All lanes : Anti-RNF169 antibody (ab188237) at 1/2000 dilution

    Lane 1 : U2OS cell lysate (si RNA control)
    Lane 2 : U2OS cell lysate (si RNA RNF169 transfected)
    Lane 3 : NIH-3T3 cell lysate
    Lane 4 : INS-1 cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 77 kDa

References

This product has been referenced in:

  • R Menon V  et al. DYRK1A regulates the recruitment of 53BP1 to the sites of DNA damage in part through interaction with RNF169. Cell Cycle 18:531-551 (2019). WB ; Human . Read more (PubMed: 30773093) »
See 1 Publication for this product

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