Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF169
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 216 - 265 (TETGKRKMDE QKKRDEPLVL KTNLERCPAR LSDSENEEPS RGQMTQTHRS) of human RNF169 (NP_001092108).

  • Positive control

    • THP-1 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by peptide affinity chromatography method.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RNF169 family.
    Contains 1 RING-type zinc finger.
  • Domain

    The MIU motif (motif interacting with ubiquitin) mediates the interaction with both 'Lys-48'- and 'Lys-63'-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction with ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833).
  • Cellular localization

    Nucleus > nucleoplasm. Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase RNF169 antibody
    • KIAA1991 antibody
    • RING finger protein 169 antibody
    • RN169_HUMAN antibody
    • RNF169 antibody
    see all

Images

  • Anti-RNF169 antibody (ab87711) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP-1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size: 77 kDa
    Observed band size: 77 kDa
    Additional bands at: 48 kDa, 66 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

References

ab87711 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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