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RecombinantRabMAb

Recombinant Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (ab181999)

Reviews (1) Submit a question References (1)
Immunoprecipitation - Anti-RNF185 antibody [EPR14070-94] (ab181999)
  • Western blot - Anti-RNF185 antibody [EPR14070-94] (ab181999)
  • Western blot - Anti-RNF185 antibody [EPR14070-94] (ab181999)
  • Anti-RNF185 antibody [EPR14070-94] (ab181999)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14070-94] to RNF185 + RNF5/NG2
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94]
    See all RNF185+RNF5/NG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14070-94] to RNF185 + RNF5/NG2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and Human fetal brain lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IP
1/40.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IP
1/40.

Target

Images

  • Immunoprecipitation - Anti-RNF185 antibody [EPR14070-94] (ab181999)
    Immunoprecipitation - Anti-RNF185 antibody [EPR14070-94] (ab181999)

    Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181999 at 1/40 dilution (lane 1) or a negative control (lane 2) followed by Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Western blot - Anti-RNF185 antibody [EPR14070-94] (ab181999)
    Western blot - Anti-RNF185 antibody [EPR14070-94] (ab181999)
    All lanes : Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (ab181999) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

    Predicted band size: 20 kDa

  • Western blot - Anti-RNF185 antibody [EPR14070-94] (ab181999)
    Western blot - Anti-RNF185 antibody [EPR14070-94] (ab181999)
    All lanes : Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (ab181999) at 1/10000 dilution

    Lane 1 : Human RNF185 full-length ORF recombinant protein with GST-tag at N-terminal, with 5% NFDM/TBST
    Lane 2 : Human RNF5 full-length ORF recombinant protein with GST-tag at N-terminal with 5% NFDM/TBST

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 20 kDa


    Exposure time: 1 second
  • Anti-RNF185 antibody [EPR14070-94] (ab181999)
    Anti-RNF185 antibody [EPR14070-94] (ab181999)

References (1)

Publishing research using ab181999? Please let us know so that we can cite the reference in this datasheet.

ab181999 has been referenced in 1 publication.

  • van de Weijer ML  et al. Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. Mol Cell 79:768-781.e7 (2020). PubMed: 32738194

Customer reviews and Q&As

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Western blot abreview for Anti-RNF185 antibody [EPR14070-94]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Nov 10 2020

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