Overview

  • Product name

  • Description

    Mouse polyclonal to RNF185
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human RNF185 protein (NP_689480)

  • Positive control

    • RNF185 transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, Whole serum, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate
  • Concentration information loading...
  • Purity

    Purified IgA
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.

Target

  • Relevance

    RNF185 contains 1 RING-type zinc finger. The exact function of RNF185 remains unknown. There are 2 named isoforms produced by alternative splicing.
  • Cellular localization

    Mitochondrion outer membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase RNF185 antibody
    • FLJ38628 antibody
    • Ring finger protein 185 antibody

Images

  • All lanes : Anti-RNF185 antibody (ab73118) at 1/500 dilution

    Lane 1 : RNF185 transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size: 20 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?

References

ab73118 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab73118.
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