Key features and details
- Mouse polyclonal to RNF185
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF185 antibody
See all RNF185 primary antibodies
DescriptionMouse polyclonal to RNF185
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length human RNF185 protein (NP_689480)
- RNF185 transfected 293T cell lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, Whole serum, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate
Concentration information loading...
Our Abpromise guarantee covers the use of ab73118 in the following tested applications.
|WB||1/500 - 1/1000. Predicted molecular weight: 20 kDa.|
RelevanceRNF185 contains 1 RING-type zinc finger. The exact function of RNF185 remains unknown. There are 2 named isoforms produced by alternative splicing.
Cellular localizationMitochondrion outer membrane; Multi-pass membrane protein.
- E3 ubiquitin protein ligase RNF185 antibody
- FLJ38628 antibody
- Ring finger protein 185 antibody
All lanes : Anti-RNF185 antibody (ab73118) at 1/500 dilution
Lane 1 : RNF185 transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 20 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
ab73118 has not yet been referenced specifically in any publications.