Recombinant
RabMAb

Anti-RNF185 antibody [EPR14070-94] (ab181999)

Overview

  • Product name
    Anti-RNF185 antibody [EPR14070-94]
    See all RNF185 primary antibodies
  • Description
    Rabbit monoclonal [EPR14070-94] to RNF185
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human RNF185 aa 1-150. The exact sequence is proprietary.
    Database link: Q96GF1

  • Positive control
    • HeLa and Human fetal brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14070-94
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IP 1/40.

Target

Images

  • Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181999 at 1/40 dilution (lane 1) or a negative control (lane 2) followed by Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • All lanes : Anti-RNF185 antibody [EPR14070-94] (ab181999) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

    Predicted band size: 20 kDa

References

ab181999 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181999.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up