Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceRNF186 contains a RING type zinc finger, a motif known to be involved in protein-protein and protein-DNA interactions. The specific function of this protein is unknown.
Cellular localizationMembrane; Multi pass membrane protein.
- FLJ20225 antibody
- Ring finger protein 186 antibody
- RNF 186 antibody
- RP11 91K11.1 antibody
Anti-RNF186 antibody (ab86546) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 24 kDa
Observed band size: ~24 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab86546 has not yet been referenced specifically in any publications.