Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF186
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 108-157 (QLAQPCTEVS LCPQGLVDPA DLAAGHPSLV GEDGQDEVSA NHVAARRLAA) of human RNF186 (NP_061935).

  • Positive control

    • Transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by peptide affinity chromatography method.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance

    RNF186 contains a RING type zinc finger, a motif known to be involved in protein-protein and protein-DNA interactions. The specific function of this protein is unknown.
  • Cellular localization

    Membrane; Multi pass membrane protein.
  • Database links

  • Alternative names

    • FLJ20225 antibody
    • Ring finger protein 186 antibody
    • RNF 186 antibody
    • RP11 91K11.1 antibody

Images

  • Anti-RNF186 antibody (ab86547) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa

References

ab86547 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting us.
I am sorry to hear that the antibody is not working as expected.

Yes, I'd be happy to replace, credit or refund the antibody. Which option would you prefer?

Could you please let me know a few more details for our internal records:

1) The expressed RNF186 protein is of which species (human?)?
2) How much protein did you load?
3) Is the secondary antibody working well otherwise?

I look forward to hear back from you.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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