Key features and details
- Rabbit polyclonal to RNF20
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF20 antibody
See all RNF20 primary antibodies
DescriptionRabbit polyclonal to RNF20
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human RNF20.
(Peptide available as
- This antibody gave a positive signal in Human hTERT fibroblasts whole cell lysate within Western blot and HeLa whole cell lysate within Immunofluorescence.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab33500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use with methanol fixed cells. This antibody does not appear to be compatible with paraformaldehyde fixation.
ab33500 can show some non-specific bands - anyone wanting to use this antibody for WB should consider image below or abreview.
FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family.
Contains 1 RING-type zinc finger.
- Information by UniProt
- BRE1 A antibody
- BRE1 antibody
- BRE1 E3 ubiquitin ligase homolog antibody
ab33500 (1/500) detecting RNF20 in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
All lanes : Anti-RNF20 antibody (ab33500) at 1/500 dilution
Lane 1 : 20 µg Human hTERT fibroblasts whole cell lysate
Lane 2 : 20 µg Human hTERT fibroblasts whole cell lysate transfected with RNF20 siRNA (3 days)
All lanes : Goat anti-Rabbit conjugated to HRP at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible non-specific binding), 170 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)
ab33500 has been referenced in 1 publication.
- Soo Lee N et al. TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage. Nat Commun 7:10463 (2016). PubMed: 26781088