Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RNF20.
    (Peptide available as ab27758)

  • Positive control

    • This antibody gave a positive signal in Human hTERT fibroblasts whole cell lysate within Western blot and HeLa whole cell lysate within Immunofluorescence.

Properties

Applications

Our Abpromise guarantee covers the use of ab33500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

Use with methanol fixed cells. This antibody does not appear to be compatible with paraformaldehyde fixation.

WB 1/500.

ab33500 can show some non-specific bands - anyone wanting to use this antibody for WB should consider image below or abreview.

Target

  • Function

    Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • Tissue specificity

    Expressed in the normal brain and also in malignant gliomas (at protein level).
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

Images

  • ab33500 (1/500) detecting RNF20 in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • All lanes : Anti-RNF20 antibody (ab33500) at 1/500 dilution

    Lane 1 : 20 µg Human hTERT fibroblasts whole cell lysate
    Lane 2 : 20 µg Human hTERT fibroblasts whole cell lysate transfected with RNF20 siRNA (3 days)

    Secondary
    All lanes : Goat anti-Rabbit conjugated to HRP at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 120 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 150 kDa (possible non-specific binding), 170 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)

    See Abreview

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
25 µg
Specification
U2OS
Treatment
Control or RNF40 (not RNF20) siRNA for 72 h.
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Prof. Steven Johnsen

Verified customer

Submitted Aug 08 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (hTERT fibroblasts)
Loading amount
30 µg
Specification
hTERT fibroblasts
Treatment
RNF20 siRNA for 3 days
Gel Running Conditions
Reduced Denaturing (7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 15 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 19 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231 and HeLa Cells)
Specification
MDA-MB-231 and HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 24 2008

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