Product nameAnti-RNF20 antibody [EPR13563(B)]
See all RNF20 primary antibodies
DescriptionRabbit monoclonal [EPR13563(B)] to RNF20
Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human RNF20 aa 500-600 (Cysteine residue). The exact sequence is proprietary.
Database link: Q5VTR2
- 293T, HeLa, Jurkat, and MCF7 cell lysates. Jurkat cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 114 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family.
Contains 1 RING-type zinc finger.
- Information by UniProt
- BRE1 A antibody
- BRE1 antibody
- BRE1 E3 ubiquitin ligase homolog antibody
All lanes : Anti-RNF20 antibody [EPR13563(B)] (ab181032) at 1/10000 dilution
Lane 1 : 293T lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 114 kDa
Flow cytometric analysis of permeabilized Jurkat cells labeling RNF20 with ab181032 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from 293 cell lysate labeling RNF20 with ab181032 at 1/10 dilution.
ab181032 has not yet been referenced specifically in any publications.