Overview

  • Product name

    Anti-RNF20 antibody [EPR13563(B)]
    See all RNF20 primary antibodies
  • Description

    Rabbit monoclonal [EPR13563(B)] to RNF20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RNF20 aa 500-600 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q5VTR2

  • Positive control

    • 293T, HeLa, Jurkat, and MCF7 cell lysates. Jurkat cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13563(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 114 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
    • Tissue specificity

      Expressed in the normal brain and also in malignant gliomas (at protein level).
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the BRE1 family.
      Contains 1 RING-type zinc finger.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • BRE1 A antibody
      • BRE1 antibody
      • BRE1 E3 ubiquitin ligase homolog antibody
      • BRE1-A antibody
      • BRE1A antibody
      • BRE1A_HUMAN antibody
      • E3 ubiquitin-protein ligase BRE1A antibody
      • hBRE1 antibody
      • Homolog of S. cerevisiae BRE1 antibody
      • RING finger protein 20 antibody
      • Ring finger protein 20 E3 ubiquitin protein ligase antibody
      • Rnf20 antibody
      see all

    Images

    • All lanes : Anti-RNF20 antibody [EPR13563(B)] (ab181032) at 1/10000 dilution

      Lane 1 : 293T lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 114 kDa

    • Flow cytometric analysis of permeabilized Jurkat cells labeling  RNF20 with ab181032 (red) at 1/10 dilution  or a rabbit IgG (negative) (green).

    • Western blot analysis on immunoprecipitation pellet from 293 cell lysate labeling RNF20 with ab181032 at 1/10 dilution.

    References

    ab181032 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab181032.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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