Key features and details
- Rabbit monoclonal [EPR13563(B)] to RNF20 - BSA and Azide free
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Mouse, Human
Product nameAnti-RNF20 antibody [EPR13563(B)] - BSA and Azide free
See all RNF20 primary antibodies
DescriptionRabbit monoclonal [EPR13563(B)] to RNF20 - BSA and Azide free
Tested applicationsSuitable for: IP, Flow Cyt, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human RNF20 aa 500-600 (Cysteine residue). The exact sequence is proprietary.
Database link: Q5VTR2
ab250297 is the carrier-free version of ab181032 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab250297 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab250297 in the following tested applications.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 114 kDa.|
FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family.
Contains 1 RING-type zinc finger.
- Information by UniProt
- BRE1 A antibody
- BRE1 antibody
- BRE1 E3 ubiquitin ligase homolog antibody
All lanes : Anti-RNF20 antibody [EPR13563(B)] (ab181032) at 1/10000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 114 kDa
This data was developed using ab181032, the same antibody clone in a different buffer formulation.
ab250297 has not yet been referenced specifically in any publications.