Overview

  • Product name
    Anti-RNF20 antibody - N-terminal
    See all RNF20 primary antibodies
  • Description
    Rabbit polyclonal to RNF20 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 19 amino acid synthetic peptide derived from near the N terminus of Human RNF20 (NP_062538).

  • Positive control
    • Human liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab167086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 114 kDa.

Target

  • Function
    Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • Tissue specificity
    Expressed in the normal brain and also in malignant gliomas (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

Images

  • Lane 1 : Anti-RNF20 antibody - N-terminal (ab167086) at 1 µg/ml
    Lane 2 : Anti-RNF20 antibody - N-terminal (ab167086) at 2 µg/ml

    All lanes : Human liver tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 114 kDa

References

ab167086 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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