• Product name

  • Description

    Rabbit polyclonal to RNF216
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human RNF216 aa 100-150. The exact sequence is proprietary. (NP_996999.1).
    Database link: Q9NWF9

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate (ab150035).
  • General notes

    Protein previously labeled as TRIAD3.



Our Abpromise guarantee covers the use of ab226215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 99 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Relevance

    The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
  • Cellular localization

  • Database links

  • Alternative names

    • CAHH antibody
    • E3 ubiquitin ligase TRIAD3 antibody
    • E3 ubiquitin-protein ligase RNF216 antibody
    • ring finger protein 216 antibody
    • RNF216 antibody
    • Triad domain-containing protein 3 antibody
    • TRIAD3 antibody
    • U7I1 antibody
    • UBCE7IP1 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 1 antibody
    • UiP83 antibody
    • ZIN antibody
    • Zinc finger protein inhibiting NF kappa B antibody
    see all


  • All lanes : Anti-RNF216 antibody (ab226215) at 0.4 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 99 kDa

    Exposure time: 3 minutes
  • RNF216 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226215 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226215 at 1 µg/ml.

    Lane 1: ab226215 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.


ab226215 has not yet been referenced specifically in any publications.

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