• Product name

  • Description

    Rabbit polyclonal to RNF216
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse RNF216 (N terminal).
    (Peptide available as ab39886)

  • Positive control

    • Mouse heart cell lysate
  • General notes

    Protein previously labeled as TRIAD3.



Our Abpromise guarantee covers the use of ab25961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).Can be blocked with Human RNF216 peptide (ab39886).


  • Relevance

    The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
  • Cellular localization

  • Database links

  • Alternative names

    • CAHH antibody
    • E3 ubiquitin ligase TRIAD3 antibody
    • E3 ubiquitin-protein ligase RNF216 antibody
    • ring finger protein 216 antibody
    • RNF216 antibody
    • Triad domain-containing protein 3 antibody
    • TRIAD3 antibody
    • U7I1 antibody
    • UBCE7IP1 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 1 antibody
    • UiP83 antibody
    • ZIN antibody
    • Zinc finger protein inhibiting NF kappa B antibody
    see all


  • Lane 1 : Anti-RNF216 antibody (ab25961) at 0.5 µg/ml
    Lane 2 : Anti-RNF216 antibody (ab25961) at 1 µg/ml
    Lane 3 : Anti-RNF216 antibody (ab25961) at 2 µg/ml

    All lanes : Mouse heart cell lysates

    Predicted band size: 99 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?

  • ab25961 at 10µg/ml staining RNF216 in mouse heart by IHC 

  • Immunofluorescence of RNF216 in Mouse Heart cells using ab25961 at 20 ug/ml.


This product has been referenced in:

  • Rajagopalan S  et al. TNFR-associated factor 6 and TGF-ß-activated kinase 1 control signals for a senescence response by an endosomal NK cell receptor. J Immunol 192:714-21 (2014). Read more (PubMed: 24337384) »
  • Xu C  et al. Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination. Autophagy 10:2239-50 (2014). Read more (PubMed: 25484083) »
See all 3 Publications for this product

Customer reviews and Q&As


Thank you for contacting Abcam earlier today.

As we discussed on the telephone I am going to send you ab22058 to try out alongside ab25961 to see if the 130kDa band that you are seeing is the correct band or not. Your new order number is ****** and you should receive it early next week.

Please let me know if there is anything else I can help you with.

Read More

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