Key features and details
- Rabbit polyclonal to RNF216 - N-terminal
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RNF216 antibody - N-terminal
See all RNF216 primary antibodies
DescriptionRabbit polyclonal to RNF216 - N-terminal
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Mouse RNF216 (N terminal).
Database link: P58283
- Human testis tissue, mouse heart cells and mouse heart cell lysate.
Protein previously labeled as TRIAD3.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab189113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 97 kDa.|
RelevanceThe TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
- CAHH antibody
- E3 ubiquitin ligase TRIAD3 antibody
- E3 ubiquitin-protein ligase RNF216 antibody
Lane 1 : Anti-RNF216 antibody - N-terminal (ab189113) at 0.5 µg/ml
Lane 2 : Anti-RNF216 antibody - N-terminal (ab189113) at 1 µg/ml
Lane 3 : Anti-RNF216 antibody - N-terminal (ab189113) at 2 µg/ml
All lanes : mouse heart cell lysate
Predicted band size: 97 kDa
Immunohistochemical analysis of paraffin embedded formalin fixed human testis tissue labeling RNF216 using ab189113 at 5 µg/ml.
Immunofluorescent analysis of mouse heart cells labeling RNF216 with ab189113 at 20 ug/ml.
ab189113 has not yet been referenced specifically in any publications.