Overview

  • Product name

    Anti-RNF216 antibody - N-terminal
    See all RNF216 primary antibodies
  • Description

    Rabbit polyclonal to RNF216 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse RNF216 (N terminal).
    Database link: P58283

  • Positive control

    • Human testis tissue, mouse heart cells and mouse heart cell lysate.
  • General notes

    Protein previously labeled as TRIAD3.

Properties

Applications

Our Abpromise guarantee covers the use of ab189113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 97 kDa.

Target

  • Relevance

    The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • CAHH antibody
    • E3 ubiquitin ligase TRIAD3 antibody
    • E3 ubiquitin-protein ligase RNF216 antibody
    • ring finger protein 216 antibody
    • RNF216 antibody
    • Triad domain-containing protein 3 antibody
    • TRIAD3 antibody
    • U7I1 antibody
    • UBCE7IP1 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 1 antibody
    • UiP83 antibody
    • ZIN antibody
    • Zinc finger protein inhibiting NF kappa B antibody
    see all

Images

  • Lane 1 : Anti-RNF216 antibody - N-terminal (ab189113) at 0.5 µg/ml
    Lane 2 : Anti-RNF216 antibody - N-terminal (ab189113) at 1 µg/ml
    Lane 3 : Anti-RNF216 antibody - N-terminal (ab189113) at 2 µg/ml

    All lanes : mouse heart cell lysate

    Predicted band size: 97 kDa

  • Immunohistochemical analysis of paraffin embedded formalin fixed human testis tissue labeling RNF216 using ab189113 at 5 µg/ml.

  • Immunofluorescent analysis of mouse heart cells labeling RNF216 with ab189113 at 20 ug/ml.

References

ab189113 has not yet been referenced specifically in any publications.

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