Key features and details
- Goat polyclonal to RNF22
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF22 antibody
See all RNF22 primary antibodies
DescriptionGoat polyclonal to RNF22
Specificityab115606 is expected to recognise all three human isoforms of this protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog
- Human Adrenal tissue; Mouse brain lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab115606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 81 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProbably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Tissue specificityExpressed in brain, heart, uterus and testis.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger.
DomainThe interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Cellular localizationCytoplasm. Early endosome.
- Information by UniProt
- BERP antibody
- Brain expressed ring finger antibody
- Brain-expressed RING finger protein antibody
Anti-RNF22 antibody (ab115606) at 1 µg/ml + Mouse brain lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
ab115606, at 5µg/ml, staining RNF22 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115606 has not yet been referenced specifically in any publications.