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    rnf22-antibody-ab232988.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase
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Anti-RNF22 antibody (ab232988)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF22 antibody (ab232988)
  • Western blot - Anti-RNF22 antibody (ab232988)
  • Western blot - Anti-RNF22 antibody (ab232988)
  • Western blot - Anti-RNF22 antibody (ab232988)
  • Western blot - Anti-RNF22 antibody (ab232988)

Key features and details

  • Rabbit polyclonal to RNF22
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RNF22 antibody
    See all RNF22 primary antibodies
  • Description

    Rabbit polyclonal to RNF22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Cow, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human RNF22 aa 2-152. Two N-terminal tags. (Expressed in E.coli).
    Sequence:

    AKREDSPGPEVQPMDKQFLVCSICLDRYQCPKVLPCLHTFCERCLQNYIP AQSLTLSCPVCRQTSILPEQGVSALQNNFFISSLMEAMQQAPDGAHDPED PHPLSVVAGRPLSCPNHEGKTMEFYCEACETAMCGECRAGEHREHGTVLL R


    Database link: O75382
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant human RNF22 protein; Rat and cow cerebrum tissue lysate; Human serum. IHC-P: Human brain tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Rat brain cerebrum tissue lysate - total protein (ab4033)

Applications

Our Abpromise guarantee covers the use of ab232988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 80 kDa.

Target

  • Function

    Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificity

    Expressed in brain, heart, uterus and testis.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Domain

    The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
  • Cellular localization

    Cytoplasm. Early endosome.
  • Target information above from: UniProt accession O75382 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 534510 Cow
    • Entrez Gene: 10612 Human
    • Entrez Gene: 55992 Mouse
    • Entrez Gene: 83616 Rat
    • Omim: 605493 Human
    • SwissProt: O75382 Human
    • SwissProt: Q9R1R2 Mouse
    • SwissProt: O70277 Rat
    • Unigene: 591992 Human
    • Unigene: 29290 Mouse
    • Unigene: 203982 Rat
    see all
  • Alternative names

    • BERP antibody
    • Brain expressed ring finger antibody
    • Brain-expressed RING finger protein antibody
    • FLJ16135 antibody
    • HAC1 antibody
    • RING finger protein 22 antibody
    • RING finger protein 97 antibody
    • RNF22 antibody
    • RNF97 antibody
    • Trim3 antibody
    • TRIM3_HUMAN antibody
    • Tripartite motif containing 3 antibody
    • Tripartite motif protein TRIM3 antibody
    • Tripartite motif-containing protein 3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF22 antibody (ab232988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF22 antibody (ab232988)

    Formalin-fixed, paraffin-embedded human brain tissue stained for RNF22 at 20μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-RNF22 antibody (ab232988)
    Western blot - Anti-RNF22 antibody (ab232988)
    Anti-RNF22 antibody (ab232988) at 2 µg/ml + Recombinant human RNF22

    Developed using the ECL technique.

    Predicted band size: 80 kDa

  • Western blot - Anti-RNF22 antibody (ab232988)
    Western blot - Anti-RNF22 antibody (ab232988)
    Anti-RNF22 antibody (ab232988) at 2 µg/ml + Rat cerebrum tissue lysate

    Developed using the ECL technique.

    Predicted band size: 80 kDa

  • Western blot - Anti-RNF22 antibody (ab232988)
    Western blot - Anti-RNF22 antibody (ab232988)
    Anti-RNF22 antibody (ab232988) at 2 µg/ml + Cow cerebrum tissue lysate

    Developed using the ECL technique.

    Predicted band size: 80 kDa

  • Western blot - Anti-RNF22 antibody (ab232988)
    Western blot - Anti-RNF22 antibody (ab232988)
    Anti-RNF22 antibody (ab232988) at 1 µg/ml + Human serum

    Developed using the ECL technique.

    Predicted band size: 80 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab232988? Please let us know so that we can cite the reference in this datasheet.

    ab232988 has not yet been referenced specifically in any publications.

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