Key features and details
- Mouse polyclonal to RNF220
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF220 antibody
See all RNF220 primary antibodies
DescriptionMouse polyclonal to RNF220
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Full length human RNF220 protein (AAH98300.1)
- RNF220 transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab69357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 63 kDa).|
RelevanceRNF220 contains one RING-type zinc finger. There are two named isoforms.
- C1orf164 antibody
- Chromosome 1 open reading frame 164 antibody
- FLJ10597 antibody
All lanes : Anti-RNF220 antibody (ab69357) at 1/500 dilution
Lane 1 : RNF220 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 63 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab69357 at 10µg/ml staining RNF220 in Hela cells by ICC/IF.
ab69357 has not yet been referenced specifically in any publications.