Product nameAnti-RNF25 antibody
See all RNF25 primary antibodies
DescriptionRabbit polyclonal to RNF25
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within amino acids 409-459 of Human RNF25 (NP_071898.2).
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140514 was affinity purified using an epitope specific to RNF25 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab140514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 51 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
Contains 1 RWD domain.
DomainThe RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
- Information by UniProt
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
All lanes : Anti-RNF25 antibody (ab140514) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 seconds
Immunoprecipitation of RNF25 from HeLa whole cell lysate using ab140514 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
For blotting immunoprecipitated RNF25, ab140514 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab140514 has not yet been referenced specifically in any publications.