Key features and details
- Rabbit polyclonal to RNF25
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RNF25 antibody
See all RNF25 primary antibodies
DescriptionRabbit polyclonal to RNF25
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Our Abpromise guarantee covers the use of ab51475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).|
|IP||Use a concentration of 1 µg/ml.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
Contains 1 RWD domain.
DomainThe RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
- Information by UniProt
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution
Lane 1 : HEK293 lysate from
cells transfected with human
Lane 2 : HEK293 lysate from
cells transfected with mouse RNF25
Lane 3 : HEK293 lysate from cells transfected with empty vector
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
1ug ab51475 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.
ab51475 has not yet been referenced specifically in any publications.