Anti-RNF25 antibody (ab89281)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-RNF25 antibody
See all RNF25 primary antibodies -
Description
Mouse polyclonal to RNF25 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length Human RNF25 protein (NP_071898.2).
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Positive control
- HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab89281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger.
Contains 1 RWD domain. -
Domain
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination. -
Post-translational
modificationsUbiquitinated. - Information by UniProt
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Database links
- Entrez Gene: 64320 Human
- SwissProt: Q96BH1 Human
- Unigene: 471403 Human
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Alternative names
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
see all
Images
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Western blot - Anti-RNF25 antibody (ab89281)Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Western blot - Anti-RNF25 antibody (ab89281)Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Western blot - Anti-RNF25 antibody (ab89281)All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml
Lane 1 : RNF25 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Cho JH et al. RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-?B-mediated ERK reactivation. Cell Death Dis 9:587 (2018). Read more (PubMed: 29789542) »
