Anti-RNF25 antibody (ab89281)
Key features and details
- Mouse polyclonal to RNF25
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF25 antibody
See all RNF25 primary antibodies -
Description
Mouse polyclonal to RNF25 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length Human RNF25 protein (NP_071898.2).
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Positive control
- HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger.
Contains 1 RWD domain. -
Domain
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination. -
Post-translational
modificationsUbiquitinated. - Information by UniProt
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Database links
- Entrez Gene: 64320 Human
- SwissProt: Q96BH1 Human
- Unigene: 471403 Human
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Alternative names
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
see all
Images
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Western blot - Anti-RNF25 antibody (ab89281)Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Western blot - Anti-RNF25 antibody (ab89281)Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Western blot - Anti-RNF25 antibody (ab89281)All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml
Lane 1 : RNF25 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (1)
ab89281 has been referenced in 1 publication.
- Cho JH et al. RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-?B-mediated ERK reactivation. Cell Death Dis 9:587 (2018). PubMed: 29789542
