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    rnf25-antibody-ab89281.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase
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Anti-RNF25 antibody (ab89281)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-RNF25 antibody (ab89281)
  • Western blot - Anti-RNF25 antibody (ab89281)
  • Western blot - Anti-RNF25 antibody (ab89281)

Key features and details

  • Mouse polyclonal to RNF25
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-RNF25 antibody
    See all RNF25 primary antibodies
  • Description

    Mouse polyclonal to RNF25
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length Human RNF25 protein (NP_071898.2).

  • Positive control

    • HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab89281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • Domain

    The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • Post-translational
    modifications

    Ubiquitinated.
  • Target information above from: UniProt accession Q96BH1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64320 Human
    • SwissProt: Q96BH1 Human
    • Unigene: 471403 Human
    • Alternative names

      • AO7 antibody
      • E3 ubiquitin protein ligase RNF25 antibody
      • E3 ubiquitin-protein ligase RNF25 antibody
      • EC 6.3.2.- antibody
      • FLJ13906 antibody
      • RING finger protein 25 antibody
      • RING finger protein AO7 antibody
      • RNF 25 antibody
      • RNF25 antibody
      • RNF25_HUMAN antibody
      see all

    Images

    • Western blot - Anti-RNF25 antibody (ab89281)
      Western blot - Anti-RNF25 antibody (ab89281)
      Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg

      Predicted band size: 51 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-RNF25 antibody (ab89281)
      Western blot - Anti-RNF25 antibody (ab89281)
      Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg

      Predicted band size: 51 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-RNF25 antibody (ab89281)
      Western blot - Anti-RNF25 antibody (ab89281)
      All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml

      Lane 1 : RNF25 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 51 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?
      Additional bands at: 45 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab89281? Please let us know so that we can cite the reference in this datasheet.

    ab89281 has been referenced in 1 publication.

    • Cho JH  et al. RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-?B-mediated ERK reactivation. Cell Death Dis 9:587 (2018). PubMed: 29789542

    Customer reviews and Q&As

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