Key features and details
- Rabbit polyclonal to RNF31/HOIP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF31/HOIP antibody
See all RNF31/HOIP primary antibodies
DescriptionRabbit polyclonal to RNF31/HOIP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 1022-1072 of Human RNF31/HOIP (NP_060469.4).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 120 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionE3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
Tissue specificityExpressed in both normal and transformed breast epithelial cell lines.
Sequence similaritiesContains 2 IBR-type zinc fingers.
Contains 3 RanBP2-type zinc fingers.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain.
DomainThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
- Information by UniProt
- E3 ubiquitin protein ligase RNF31 antibody
- FLJ10111 antibody
- FLJ23501 antibody
All lanes : Anti-RNF31/HOIP antibody (ab125189) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 3 minutes
Detection of human RNF31/HOIP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF31/HOIP antibody ab125189 used for IP at 6 µg per reaction. RNF31/HOIP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated RNF31/HOIP, ab125189 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
ab125189 has been referenced in 2 publications.