Key features and details
- Rabbit polyclonal to RNF31/HOIP
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF31/HOIP antibody
See all RNF31/HOIP primary antibodies
DescriptionRabbit polyclonal to RNF31/HOIP
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human kidney tissue; ICC: PC-3 whole cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionE3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
Tissue specificityExpressed in both normal and transformed breast epithelial cell lines.
Sequence similaritiesContains 2 IBR-type zinc fingers.
Contains 3 RanBP2-type zinc fingers.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain.
DomainThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
- Information by UniProt
- E3 ubiquitin protein ligase RNF31 antibody
- FLJ10111 antibody
- FLJ23501 antibody
Immunohistochemical analysis of paraffin embedded human kidney tissue labeling RNF31/HOIP in the cytoplasm and membrane of cells in tubules with ab187976 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemical analysis of PC-3 (Human prostate adenocarcinoma cell line) whole cells labeling RNF31/HOIP in the cytosol with ab187976 at 2 µg/ml.
ab187976 has not yet been referenced specifically in any publications.