Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 123 kDa.Can be blocked with Human RNF31/HOIP peptide (ab22938).|
FunctionE3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
Tissue specificityExpressed in both normal and transformed breast epithelial cell lines.
Sequence similaritiesContains 2 IBR-type zinc fingers.
Contains 3 RanBP2-type zinc fingers.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain.
DomainThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
- Information by UniProt
- E3 ubiquitin protein ligase RNF31 antibody
- FLJ10111 antibody
- FLJ23501 antibody
Anti-RNF31/HOIP antibody (ab2919) at 2 µg/ml + HepG2 lysate (RIPA buffer, 30µg total protein per lane).
Predicted band size: 123 kDa
ab2919 staining (2
µg/ml) of HepG2 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
This product has been referenced in:
- Wang L et al. The Linear Ubiquitin Assembly Complex Modulates Latent Membrane Protein 1 Activation of NF-?B and Interferon Regulatory Factor 7. J Virol 91:N/A (2017). Read more (PubMed: 27903798) »
- Culver C et al. Mechanism of hypoxia-induced NF-kappaB. Mol Cell Biol 30:4901-21 (2010). WB ; Human . Read more (PubMed: 20696840) »