Product nameAnti-RNF31/HOIP antibody
See all RNF31/HOIP primary antibodies
DescriptionRabbit polyclonal to RNF31/HOIP
Tested applicationsSuitable for: ICC/IF, WB, ChIPmore details
Species reactivityReacts with: Mouse, Human
- ab46322 gave a positive signal in Jurkat Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab46322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250. Detects a band of approximately 116 kDa (predicted molecular weight: 119 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionE3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
Tissue specificityExpressed in both normal and transformed breast epithelial cell lines.
Sequence similaritiesContains 2 IBR-type zinc fingers.
Contains 3 RanBP2-type zinc fingers.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain.
DomainThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
- Information by UniProt
- E3 ubiquitin protein ligase RNF31 antibody
- FLJ10111 antibody
- FLJ23501 antibody
Anti-RNF31/HOIP antibody (ab46322) at 1/250 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible isoform), 50 kDa (possible isoform), 60 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab46322 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 cells at 5µg/ml.
Western blot analysis of A549 cell lysate (100μg/lane) RNF31/HOIP with ab46322 at 1/1000. A HRP-conjugated Goat anti-rabbit polyclonal (1/10000) was used as the secondary antibody.
lane 1-2 : siCT A549
lane 3-4 : siHOIP A549
This product has been referenced in:
- Dziedzic SA et al. ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC. Nat Cell Biol 20:58-68 (2018). Read more (PubMed: 29203883) »
- Qiu P et al. MicroRNA-378 regulates cell proliferation and migration by repressing RNF31 in pituitary adenoma. Oncol Lett 15:789-794 (2018). Read more (PubMed: 29399147) »