Anti-RNF34 antibody (ab169047)
Key features and details
- Mouse polyclonal to RNF34
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF34 antibody
See all RNF34 primary antibodies -
Description
Mouse polyclonal to RNF34 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Orangutan
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Immunogen
Full length protein, corresponding to amino acids 1-373 of Human RNF34 Isoform 2 (NP_919247.1).
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Positive control
- RNF34 transfected 293T cell lysate; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab169047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219). -
Tissue specificity
Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 FYVE-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 2 SAP domains. -
Domain
The RING-type zinc finger is required for the ubiquitination of target proteins.
The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids. -
Post-translational
modificationsAutoubiquitinated (in vitro).
Proteolytically cleaved by caspases upon induction of apoptosis by TNF. -
Cellular localization
Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol. - Information by UniProt
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Database links
- Entrez Gene: 506764 Cow
- Entrez Gene: 80196 Human
- Entrez Gene: 282845 Rat
- Omim: 608299 Human
- SwissProt: Q5E9J6 Cow
- SwissProt: Q969K3 Human
- SwissProt: Q5NVC7 Orangutan
- SwissProt: Q6AYH3 Rat
see all -
Alternative names
- CARP 1 antibody
- CARP-1 antibody
- CARP1 antibody
see all
Images
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Western blot - Anti-RNF34 antibody (ab169047)All lanes : Anti-RNF34 antibody (ab169047) at 1 µg/ml
Lane 1 : RNF34 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RNF34 antibody (ab169047)Immunofluorescent analysis of HeLa cells labeling RNF34 with ab169047 at 10 µg/ml.
Protocols
Datasheets and documents
References (0)
ab169047 has not yet been referenced specifically in any publications.
