Key features and details
- Mouse polyclonal to RNF34
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF34 antibody
See all RNF34 primary antibodies
DescriptionMouse polyclonal to RNF34
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Orangutan
- RNF34 transfected 293T cell lysate; HeLa cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionE3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
Tissue specificityUbiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 2 SAP domains.
DomainThe RING-type zinc finger is required for the ubiquitination of target proteins.
The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
modificationsAutoubiquitinated (in vitro).
Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
Cellular localizationCell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
- Information by UniProt
- CARP 1 antibody
- CARP-1 antibody
- CARP1 antibody
All lanes : Anti-RNF34 antibody (ab169047) at 1 µg/ml
Lane 1 : RNF34 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa
Immunofluorescent analysis of HeLa cells labeling RNF34 with ab169047 at 10 µg/ml.
ab169047 has not yet been referenced specifically in any publications.