Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF34
  • Host species

    Rabbit
  • Specificity

    Expect reactivity with isoform 1 and 2 of RNF34.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control

    • RNF34 transfected lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the given buffer.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
  • Tissue specificity

    Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • Domain

    The RING-type zinc finger is required for the ubiquitination of target proteins.
    The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
  • Post-translational
    modifications

    Autoubiquitinated (in vitro).
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
  • Cellular localization

    Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • CARP 1 antibody
    • CARP-1 antibody
    • CARP1 antibody
    • Caspase regulator CARP1 antibody
    • Caspases-8 and -10-associated RING finger protein 1 antibody
    • E3 ubiquitin-protein ligase RNF34 antibody
    • FYVE-RING finger protein Momo antibody
    • hRFI antibody
    • Human RING finger homologous to inhibitor of apoptosis protein antibody
    • RFI antibody
    • RIF antibody
    • RIFF antibody
    • RING finger protein 34 antibody
    • RING finger protein RIFF antibody
    • RNF34 antibody
    • RNF34_HUMAN antibody
    see all

Images

  • All lanes : Anti-RNF34 antibody (ab47255) at 1/1000 dilution

    Lane 1 : Empty vector 293T cell lysate
    Lane 2 : RNF34 transfected 293T cell lysate
    Lane 3 : RFFL transfected 293T cell lysate

    Lysates/proteins at 20 µg/ml per lane.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

References

ab47255 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab47255.
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