Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RNF4 aa 1-103.
    Sequence:

    MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLT CESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEEL SRD


    Database link: P78317

  • Positive control

    • IHC-P: Human skeletal muscle and placenta tissues. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab222817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Function

    E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.
  • Tissue specificity

    Widely expressed at low levels in many tissues; highly expressed in testis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates.
  • Post-translational
    modifications

    Sumoylated; conjugated by one or two SUMO1 moieties.
    Autoubiquitinated.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > PML body. Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin ligase RNF4 antibody
    • E3 ubiquitin protein ligase RNF4 antibody
    • Protein SNURF antibody
    • RES4 26 antibody
    • RING finger protein 4 antibody
    • Rnf4 antibody
    • RNF4_HUMAN antibody
    • SLX5 antibody
    • Small nuclear ring finger protein antibody
    • SNURF antibody
    see all

Images

  • Paraffin-embedded human skeletal muscle tissue stained for RNF4 using ab222817 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human placenta tissue stained for RNF4 using ab222817 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for RNF4 (green) using ab222817 at 1/100 dilution in ICC/IF. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as secondary antibody.

References

ab222817 has not yet been referenced specifically in any publications.

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