Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 4-271 of Human RNF40 (UniProt O75150).

  • Positive control

    • NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; MCF7 cells; Human colon carcinoma tissue.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 114 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes.
  • Tissue specificity

    Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 95 kDa retinoblastoma associated protein antibody
    • 95 kDa retinoblastoma protein binding protein antibody
    • 95 kDa retinoblastoma-associated protein antibody
    • BRE 1B antibody
    • BRE1 B antibody
    • BRE1 E3 ubiquitin ligase homolog B antibody
    • BRE1-B antibody
    • BRE1B antibody
    • BRE1B_HUMAN antibody
    • DKFZp686K191 antibody
    • E3 ubiquitin protein ligase BRE1B antibody
    • E3 ubiquitin-protein ligase BRE1B antibody
    • KIAA0661 antibody
    • MGC13051 antibody
    • Rb associated protein antibody
    • RBP 95 antibody
    • RBP95 antibody
    • RING finger protein 40 antibody
    • Ring finger protein 40 E3 ubiquitin protein ligase antibody
    • RNF 40 antibody
    • Rnf40 antibody
    • STARING antibody
    see all

Images

  • Anti-RNF40 antibody (ab126959) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 114 kDa



    7.5% SDS PAGE
  • ab126959, at a dilution of 1/500, staining RNF40 in paraformaldehyde-fixed MCF7 cells by Immunofluorescence. The lower image is co-stained with Hoechst 33342.
  • ab126959, at a dilution of 1/250, staining RNF40 in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Duan Y  et al. Ubiquitin ligase RNF20/40 facilitates spindle assembly and promotes breast carcinogenesis through stabilizing motor protein Eg5. Nat Commun 7:12648 (2016). Read more (PubMed: 27557628) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse colon cells)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
100000 cells
Specification
Mouse colon cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jan 27 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cell line)
Loading amount
100000 cells
Specification
Breast cell line
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 08 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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