• Product name

  • Description

    Rabbit polyclonal to RNF5/NG2
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human RNF5/NG2 aa 1-50.
    Database link: NP_008844.1

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysate (ab7899)
  • General notes

     This product was previously labelled as RNF5




Our Abpromise guarantee covers the use of ab128200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function

    Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
  • Tissue specificity

    Widely expressed.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Cellular localization

    Membrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase RNF5 antibody
    • E3 ubiquitin-protein ligase RNF5 antibody
    • G16 antibody
    • HsRma1 antibody
    • NG2 antibody
    • OTTHUMP00000029107 antibody
    • Protein G16 antibody
    • Ram1 homolog antibody
    • RING finger protein 5 antibody
    • RING5 antibody
    • RMA1 antibody
    • RNF 5 antibody
    • RNF5 antibody
    • RNF5_HUMAN antibody
    see all


  • All lanes : Anti-RNF5/NG2 antibody (ab128200) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 20 kDa

    Exposure time: 10 seconds
  • Detection of RNF5/NG2 by Western blot of immunoprecipitate

    ab128200, at 6 µg/ml, immunoprecipitating RNF5/NG2 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
    ab128200 was used at 1 µg/ml for blotting immunoprecipitated RNF5/NG2.
    Detection: Chemiluminescence with exposure time of 3 seconds.


This product has been referenced in:

  • Ramachandran S  et al. SYVN1, NEDD8, and FBXO2 Proteins Regulate ?F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Ubiquitin-mediated Proteasomal Degradation. J Biol Chem 291:25489-25504 (2016). Read more (PubMed: 27756846) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab128200.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up