Overview

  • Product name

  • Description

    Rabbit polyclonal to RNF5/NG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human RNF5/NG2 aa 1-50.
    Database link: NP_008844.1

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysate (ab7899)
  • General notes

     This product was previously labelled as RNF5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab128200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
  • Tissue specificity

    Widely expressed.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Cellular localization

    Membrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase RNF5 antibody
    • E3 ubiquitin-protein ligase RNF5 antibody
    • G16 antibody
    • HsRma1 antibody
    • NG2 antibody
    • OTTHUMP00000029107 antibody
    • Protein G16 antibody
    • Ram1 homolog antibody
    • RING finger protein 5 antibody
    • RING5 antibody
    • RMA1 antibody
    • RNF 5 antibody
    • RNF5 antibody
    • RNF5_HUMAN antibody
    see all

Images

  • All lanes : Anti-RNF5/NG2 antibody (ab128200) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 20 kDa


    Exposure time: 10 seconds
  • Detection of RNF5/NG2 by Western blot of immunoprecipitate

    ab128200, at 6 µg/ml, immunoprecipitating RNF5/NG2 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
    ab128200 was used at 1 µg/ml for blotting immunoprecipitated RNF5/NG2.
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

This product has been referenced in:

  • Ramachandran S  et al. SYVN1, NEDD8, and FBXO2 Proteins Regulate ?F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Ubiquitin-mediated Proteasomal Degradation. J Biol Chem 291:25489-25504 (2016). Read more (PubMed: 27756846) »
See 1 Publication for this product

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