Key features and details
- Rabbit polyclonal to RNF5/NG2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF5/NG2 antibody
See all RNF5/NG2 primary antibodies
DescriptionRabbit polyclonal to RNF5/NG2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human RNF5/NG2 aa 1-50.
Database link: NP_008844.1
- 293T, HeLa and Jurkat whole cell lysate (ab7899)
This product was previously labelled as RNF5
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH = 7-8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab128200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 20 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHas E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
Cellular localizationMembrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
- Information by UniProt
- E3 ubiquitin protein ligase RNF5 antibody
- E3 ubiquitin-protein ligase RNF5 antibody
- G16 antibody
All lanes : Anti-RNF5/NG2 antibody (ab128200) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 20 kDa
Exposure time: 10 seconds
Detection of RNF5/NG2 by Western blot of immunoprecipitate
ab128200, at 6 µg/ml, immunoprecipitating RNF5/NG2 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
ab128200 was used at 1 µg/ml for blotting immunoprecipitated RNF5/NG2.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab128200 has been referenced in 1 publication.
- Ramachandran S et al. SYVN1, NEDD8, and FBXO2 Proteins Regulate ?F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Ubiquitin-mediated Proteasomal Degradation. J Biol Chem 291:25489-25504 (2016). PubMed: 27756846