Anti-RNF5/NG2 antibody (ab154959)
Key features and details
- Rabbit polyclonal to RNF5/NG2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF5/NG2 antibody
See all RNF5/NG2 primary antibodies -
Description
Rabbit polyclonal to RNF5/NG2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human RNF5/NG2 aa 1-180.
Database link: Q99942 -
Positive control
- 293T whole cell lysate
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General notes
This product was previously labelled as RNF5
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab154959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 20 kDa. |
Target
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Function
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Cellular localization
Membrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles. - Information by UniProt
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Database links
- Entrez Gene: 6048 Human
- Omim: 602677 Human
- SwissProt: Q99942 Human
- Unigene: 718462 Human
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Alternative names
- E3 ubiquitin protein ligase RNF5 antibody
- E3 ubiquitin-protein ligase RNF5 antibody
- G16 antibody
see all
Images
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Western blot - Anti-RNF5/NG2 antibody (ab154959)Anti-RNF5/NG2 antibody (ab154959) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 20 kDa
12% SDS PAGE
References (0)
ab154959 has not yet been referenced specifically in any publications.
