Anti-RNF5/NG2 antibody - N-terminal (ab200389)
Key features and details
- Rabbit polyclonal to RNF5/NG2 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RNF5/NG2 antibody - N-terminal
See all RNF5/NG2 primary antibodies -
Description
Rabbit polyclonal to RNF5/NG2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human RNF5/NG2 (N terminal). NP_008844.
Database link: Q99942 -
Positive control
- Jurkat, 231 and K562 cell lysates; Human thyroid cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
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Notes |
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IHC-P
1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
Target
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Function
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Cellular localization
Membrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles. - Information by UniProt
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Database links
- Entrez Gene: 6048 Human
- Entrez Gene: 54197 Mouse
- Omim: 602677 Human
- SwissProt: Q99942 Human
- SwissProt: O35445 Mouse
- Unigene: 718462 Human
- Unigene: 274542 Mouse
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Alternative names
- E3 ubiquitin protein ligase RNF5 antibody
- E3 ubiquitin-protein ligase RNF5 antibody
- G16 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling RNF5/NG2 with ab200389 at 1/35 dilution.
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All lanes : Anti-RNF5/NG2 antibody - N-terminal (ab200389) at 1/200 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 231 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 20 kDa
Exposure time: 3 minutesGel: 10%SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab200389 has not yet been referenced specifically in any publications.