Key features and details
- Rabbit polyclonal to RNF5/NG2 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RNF5/NG2 antibody - N-terminal
See all RNF5/NG2 primary antibodies
DescriptionRabbit polyclonal to RNF5/NG2 - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human RNF5/NG2 (N terminal). NP_008844.
Database link: Q99942
- Jurkat, 231 and K562 cell lysates; Human thyroid cancer tissue.
This product was previously labelled as RNF5
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab200389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 20 kDa.|
FunctionHas E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
Cellular localizationMembrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
- Information by UniProt
- E3 ubiquitin protein ligase RNF5 antibody
- E3 ubiquitin-protein ligase RNF5 antibody
- G16 antibody
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling RNF5/NG2 with ab200389 at 1/35 dilution.
All lanes : Anti-RNF5/NG2 antibody - N-terminal (ab200389) at 1/200 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 231 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 20 kDa
Exposure time: 3 minutes
ab200389 has not yet been referenced specifically in any publications.