Overview

  • Product name

    Anti-RNF5/NG2 antibody - N-terminal
    See all RNF5/NG2 primary antibodies
  • Description

    Rabbit polyclonal to RNF5/NG2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human RNF5/NG2 (N terminal). NP_008844.
    Database link: Q99942

  • Positive control

    • Jurkat, 231 and K562 cell lysates; Human thyroid cancer tissue.
  • General notes

     This product was previously labelled as RNF5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab200389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.

Target

  • Function

    Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
  • Tissue specificity

    Widely expressed.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Cellular localization

    Membrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase RNF5 antibody
    • E3 ubiquitin-protein ligase RNF5 antibody
    • G16 antibody
    • HsRma1 antibody
    • NG2 antibody
    • OTTHUMP00000029107 antibody
    • Protein G16 antibody
    • Ram1 homolog antibody
    • RING finger protein 5 antibody
    • RING5 antibody
    • RMA1 antibody
    • RNF 5 antibody
    • RNF5 antibody
    • RNF5_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling RNF5/NG2 with ab200389 at 1/35 dilution.

  • All lanes : Anti-RNF5/NG2 antibody - N-terminal (ab200389) at 1/200 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 231 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 40 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 20 kDa


    Exposure time: 3 minutes


    Gel: 10%SDS-PAGE

References

ab200389 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab200389.
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