Key features and details
- Rabbit polyclonal to RNF6
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RNF6 antibody
DescriptionRabbit polyclonal to RNF6
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Human testis tissue; U-2 OS cells; RNF6 over-expression HEK293T cell lysate
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 78 kDa.|
FunctionE3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.
Tissue specificityWeakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in RNF6 may be a cause of esophageal cancer (ESCR) [MIM:133239]. A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage.
Sequence similaritiesBelongs to the RNF12 family.
Contains 1 RING-type zinc finger.
Developmental stageExpressed in embryo and adult testis.
Cellular localizationNucleus. Cytoplasm. Cell projection > axon. Nucleus > PML body. Localizes to the PML nuclear bodies in Sertoli cells.
- Information by UniProt
- E3 ubiquitin-protein ligase RNF6 antibody
- Ring finger protein (C3H2C3 type) 6 antibody
- Ring finger protein 6 antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue labeling RNF6 with ab204506 at 1/20 dilution.
All lanes : Anti-RNF6 antibody (ab204506) at 1/100 dilution
Lane 1 : Negative Control
Lane 2 : RNF6 over-expression HEK293T cell lysate
Developed using the ECL technique.
Predicted band size: 78 kDa
ab204506 has not yet been referenced specifically in any publications.