Key features and details
- Rabbit polyclonal to RNF7
- Suitable for: ELISA, IP, WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Purification notesThis product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab34873 in the following tested applications.
|ELISA||1/2000 - 1/10000.|
|IP||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 12.6 kDa (predicted molecular weight: 13 kDa).|
FunctionProbable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
Tissue specificityExpressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RING-box family.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
modificationsPhosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CKBBP 1 antibody
- CKBBP1 antibody
- CKII beta binding protein 1 antibody
ab34873 has been referenced in 2 publications.
- Telesio G et al. The E3 Ubiquitin Ligase RNF7 Negatively Regulates CARD14/CARMA2sh Signaling. Int J Mol Sci 18:N/A (2017). WB . PubMed: 29194363
- Chang SC & Ding JL Ubiquitination by SAG regulates macrophage survival/death and immune response during infection. Cell Death Differ N/A:N/A (2014). Flow Cyt . PubMed: 24786833